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| Subtilisin 9014-01-1 |
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| Subtilisin 9014-01-1 |
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Pharmaceutical Use |
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Subtilisin 9014-01-1
Synonyms: Subtilisin Carlsberg, Subtilopeptidase A, Bacterial Alkaline Protease
CAS #: 9014-01-1
EC #: 3.4.21.62
Sigma's Subtilisins are produced from fermentation of Bacillus licheniformis. We offer several grades and formulations including high purity lyophilized powders as well as economical industrial grade solutions.
Physical properties and Specificity:
MW: Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and a of MW 27 KDa.2
Specificity: Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.
Isoelectric point: pI = 9.4
E1%(280nm) = 8.6
Sedimentation coefficient: S20,w = 2.773
Activity Determination and Optimal Conditions:
Unit definition: One Sigma Unit will hydrolyze casein to produce color equivalent to 1.0 mmole (181 µ
g) of tyrosine per minute at pH 7.5 at 37 (color by Folin-Ciocalteu reagent).
Usage:1>Proteolytic enzymes. Mainly used in gelatin production can greatly reduce extraction time, also used in vegetable protein, animal protein and fish protein hydrolysis.
2>Bacteria fungicides. On several plant pathogenic fungi had strong antagonism, can effectively prevent the disease of cucumber and tobacco
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